Table of Contents
Definition and Background
- The sugar ribose is found in RNA; however, DNA contains the sugar deoxyribose, which is slightly different than ribose, in that it lacks an oxygen atom.
- DNA has the nucleobase thymine; whereas, RNA contains uracil. Thymine and uracil have similar base-pairing properties.
Types of RNA
Messenger RNA (mRNA)
Messenger RNA (mRNA) is transcribed from a DNA template. Coding information to the sites of the ribosomes is carried through mRNA. In mRNA, the genetic information is encoded in the sequence of nucleotides, which is arranged into codons that consist of three bases each. A specific amino acid is encoded by each one of the codons, except the stop codons, which are responsible for terminating the process of protein synthesis. Two other types of RNA are required in this process, which are ribosomal RNA (rRNA) and transfer RNA (tRNA).
Ribosomal RNA (rRNA)
Ribosomal RNA (rRNA) is the RNA component of the ribosome. The mechanism for decoding mRNA into amino acids is provided by ribosomal RNA, it also interacts with tRNAs during translation and provides peptidyl transferase activity. The necessary amino acids that correspond to the appropriate mRNA codon are brought by the tRNAs.
Transfer RNA (tRNA)
Transfer RNA (tRNA) functions in the transfer of specific active amino acids, during translation, to a growing polypeptide chain at the ribosomal site of protein synthesis. Transfer RNA contains the anticodon, which is a three-base region and can base pair to the corresponding three base codon regions on mRNA. Only one type of amino acid can be attached to each type of tRNA molecule; however, the same amino acid may be carried by tRNA molecules bearing different anticodons because multiple codons that specify the same amino acid are found in the genetic code.
Other Types of RNA
Small interfering RNA (siRNA)
Small interfering RNA (siRNA) is also known as short interfering RNA, which is a class of double-stranded RNA molecule. siRNA is involved in the RNA interference pathway. It controls the stability of the mRNA by interfering with the expression of specific genes.
Small nuclear RNA (snRNA)
snRNA molecules are either transcribed by RNA polymerase III along with all nuclear tRNAs and the 5S rRNA, or by RNA polymerase II along with mRNA. They are involved in the maintenance of the telomeres and splicing by removal of introns from pre-mRNA.
Heterogeneous nuclear RNA (hnRNA)
hnRNA is considered a single immature strand of mRNA. The two terms pre-mRNA and hnRNA are almost identical, and they are used interchangeably.
Adenine is a purine derivative nucleobase with different roles in biochemistry, including cellular respiration, in the form of ATP, NAD and FAD. It also plays a role as a chemical component of DNA and RNA in protein synthesis. Adenine has a shape that is complementary to either uracil in RNA or thymine in DNA.
Cytosine is found in both DNA and RNA, which is a pyrimidine derivative. It has a heterocyclic aromatic ring and two substituents attached which are a keto group at position 2, and an amine group at position 4). Cytidine is the nucleoside of cytosine.
Guanine is found in both RNA and DNA. It is a derivative of purine and consists of a fused pyrimidine-imidazole ring system, along with conjugated double bonds. Guanine is paired with cytosine in DNA.
Uracil is a demethylated form of thymine, and it binds to adenine, via two hydrogen bonds, in RNA. In DNA, thymine nucleobases replace uracil.
Differences between DNA and RNA
Both DNA and RNA are very similar in their structures; however, there are five main differences between them both:
|Nucleobases||Adenine, Guanine, Cytosine, Uracil||Adenine, Guanine, Cytosine, Thymine|
|Structure||Usually exists as single-stranded||
Always a double helix
|Sugar||D-ribose sugar||2′-deoxy-D-ribose pentose sugar|
|Stability||It contains a 2′-OH hydroxyl group, which makes it less stable||More stable|
RNA is usually single-stranded, and the portions are not necessarily equal
|Equal portions of Adenine-Thymine and Guanine-Cytosine nucleobases|
- From greek “kolla” (glue) “gen” (producing)
- Boiling hooves to make glue
- Gelatin — collagen that has been irreversibly hydrolyzed
- Medical uses in treating bones and skin
- Most abundant protein in body
- 25—30 % of all body protein by weight
- Synthesis decreases with age
- 29 types in the body
- Over 90 % of the collagen in the human body is type I
- Most common types — I, II, III, IV, V
- Type I collagen fibrils are stronger by weight than steel
- Basement membrane of extracellular matrix
- Blood vessel damage exposes collagen — signal to clotting system
|Collagen I||Skin, tendon, vascular ligature, organs, bone|
|Collagen III||Reticular fibers|
|Collagen IV||Basal lamina, the epithelium-secreted layer of the basement membrane|
|Collagen V||Cell surfaces, hair, and placenta|
States of collagen
- State of collagen depends on mineralization
- Bone, tendon, cartilage
- Fibrillar and non-fibrillar types
- Most abundant fibrous tissue — ligaments, tendons, skin
- Triple helix of helices
- Each polypeptide chain up to about 1,400 amino acids
- Type 1 collagen contains two identical α-1 chains and one α-2 chain
- Each chain is a left-handed helix
- Three chains coiled together to make right-handed strand (superhelix)
- Strands combined together to make fibers
Biochemistry of collagen
- Strands synthesized into endoplasmic reticulum (ER)
- Hydroxylation of prolines and lysines occurs in ER
- Hydroxylation necessary for collagen secretion
- Secreted collagen strands form helix
- Helices cross-link for strength
- Proline hydroxylation
- Requires vitamin C
- Deficiency leads to scurvy
- Crusaders of 13th century suffered from scurvy
- Value of citrus fruits for preventing scurvy known by Vasco de Gama´s voyage of 1497
- Nonetheless, over 2 million sailors died of scurvy between 1500 and 1800
- Proline hydroxylation increases collagen´s thermal stability
- Stabilizes collagen at body temperature
- Cooking hydrolyzed collagen — tenderizing
- Extracellular enzyme
- Oxidizes lysines/ hydroxylysines to aldehydes in extracellular matrix
- Aldehydes of allysine can react with amines of other lysines to form pyridinoline
- This cross-linking gives collagen its strength
Disease & Collagen
|Collagen vascular disease||Genetic diseases of collagen synthesis/ processing|