Antibodies are proteins that uniquely bind to a protein or other molecules. They belong to a family of globular proteins called immunoglobulins. They are antigen-reactive proteins that are present in immune serum, called antiserum. They are produced after a vertebrate host is exposed to a given antigen, called an immunogen.
The main function of an antibody is to bind to foreign molecules. Most antibodies are structurally similar because of their Y-shaped molecules. However, each antibody is unique because they have different amino acid sequences. This structural properties of antibodies enable it to exhibit binding versatility, binding specificity and biological activity. An individual can produce hundreds of thousands of antibodies. This enables him to detect presence of foreign or non-self molecules inside it. The Y-shaped arm of the antibody defines to which molecules they will interact. It also defines what response will be done by the cell. Possible responses include complement-mediated lysis, enhanced phagocytosis, and or occurrence of allergic reactions. These responses occur once antibodies bind to the antigens.
Humans are able express five types of antibody or immunoglobulin classes. The five classes are IgM, IgA. IgG, IgE, and IgD. These five classes may differ in their physicochemical properties which include charge, solubility and size. This variability is caused by the differences in the amino acid sequences they have. They may also differ in their serologic properties, or the way they react with antigens. The greatest factor that divides the antibodies into five classes are their differences in their behavior as antigens.
IgG is the antibody induced by protein antigens and constitutes 80% of the antibody in a serum. Human IgA, on the other hand, is the predominant class of antibody in extravascular secretions. It consists of 13% of the antibodies in human serum. Human IgM is the antibody induced by polysaccharide antigens. It is the first antibody that appears during an immune response. It is also the first ones to develop in the fetus. IgD is the antibody that remains membrane-bound. It is one of the main receptors on mature B cells. It regulates the cell’s activation. Human IgE is the antibody that binds through its Fc part of mast cells or basophils. When the mast cell and the basophil are exposed to the same antigens, it triggers allergic reactions.
Gene expression is the process by which genetic instructions are processed to produce specific gene products. Common products include proteins that are essential in different biological activities. This includes enzymes, hormones and receptors. To prevent overproduction of certain gen products, different control mechanisms are installed in the cells. Transcriptional regulators are proteins that recognizes certain areas in the DNA. When these transcriptional regulators bind to the DNA, it acts a switch that regulates transcription of distinct set of genes. Transcription switches also allows cells to respond to changes in the environment. Transcription switches may act either as repressor or activator. Repressors are proteins that switches genes off. On the other hand, activators are proteins that promotes or activates genes.
Another important function for proteins is catalyzing a number of reactions. Most of the metabolic reactions in biological systems involves the use of enzymes. In fact, these reactions will not occur if an individual do not have the enzyme to catalyze the reaction. Enzymes are highly specific that they usually catalyze a single reactions or very few related ones. Differences in the structure of the active sites of an enzyme determines it specificity to specific substrates or reactants. Enzymes enable lowering of the activation energies of biological reactions. During the reaction, enzymes undergo structural changes to not accommodate other substrate molecules. After the reaction, they will return to their original state and is ready to interact with a new substrate molecule
Different models were used to describe the action of enzymes during catalysis. The most common models are the lock and key model and the induced-fit model. In the lock and key model, Emil Fischer postulated that enzymes and substrates are like locks and keys. They fit perfectly with each other. This explains the high specificity of enzymes to its substrate because this means an enzyme will only interact with a specific substrate that fits in its active site. If the substrate and the enzyme do not fit, then no reaction will occur.
In the induced-fit model, enzyme and the substrate interacts forming a weak covalent bond. The enzyme then undergo some conformational changes that further strengthen the binding. The reaction then occur until product is formed. Changes in the conformation of the enzymes will again happen to facilitate release of the products. The enzymes then return to its original state. This model of enzyme catalysis allows can provide explanation on the action of inhibitors. Aside from the active site of the enzyme, there are other allosteric sites where other molecules may attach. Inhibitors may act on these allosteric site, and when conformational change happens, entry of the substrate to the active site will be inhibited.
Another important function of proteins is as a transporting medium for some biomolecules. Transport proteins are protein molecules that serves to move materials within an organism. Examples of transport proteins are low and high density lipoproteins, hemoglobin and serum albumin. Lipoproteins are transport proteins responsible for the transfer of hydrophobic lipids. Hemoglobin is the transport protein responsible for transporting oxygen from the lungs to the rest of the body. Albumin are carrier proteins for steroids, fatty acids and the thyroid hormone. Another type of transport proteins are the membrane transport proteins. They are responsible for the movement of ions, small molecules or other macromolecules across a biological membrane. They assist in the transport through active transport or facilitated diffusion.
Two major proteins are responsible for the transport of oxygen molecules. These are myoglobin and hemoglobin. Oxygen molecules have different affinities to these two types of proteins. Myoglobin has a higher O2 affinity even at low O2 concentration. On the other hand, hemoglobin has variable degree of O2 affinity depending on the concentration of O2. There are a number of structural similarities between myoglobin and hemoglobin. Both proteins contain a Fe atom where the O2 molecule may act as a ligand. There major difference is that myoglobin can only accommodate one oxygen molecule, while the hemoglobin can transport up to 4 oxygen molecule. An explanation to this is that, hemoglobin transports oxygen from the lungs to the muscles where the myoglobin molecules are. When they interact, oxygen will be transferred to myoglobin molecules which will be the one transferring that oxygen molecule to the muscle cells.
Na+/K+ ATPase is an enzyme that serves as a solute pump to transport sodium ions out of cells while pumping potassium ions into the cells. Transport of the ions is due to a concentration gradient. The basic mechanism of action of this transport protein involves binding of ATP to the pump before 3 intracellular Na+ ions bind also to it. ATP is then hydrolyzed, leading to phosphorylation and eventual release of ADP. Conformational changes leads to opening of the pump to the outside of the cells allowing release of the ions. Two extracellular K+ ions then bind to the pump causing dephosphorylation of the pump. The pump reverts to its original conformation and transports K+ to the inside of the cell. The process is repeated to regulate the ionic conditions inside and outside the cell.
The correct answers can be found below the references.
1. Which of the following proteins are responsible for controlling gene regulation?
- Transcription switches
2. Which transport protein can be used to determine if a patient is prone to heart diseases due to a high lipid profile?
- Membrane protein