In 1955, a cause of nonspecific vaginitis (bacterial vaginosis) was described by two scientists: Gardner and Dukes. The bug was named Haemophilus vaginalis, later the name was changed to Corynebacterium vaginale, and finally the bacterium responsible for the condition obtained the name Gardnerella Vaginalis (1980) after the scientist who discovered it.

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gardnerella vaginalis

Image: “Microscopic view of Gardnerella vaginalis, magnified 400x.” by Dr. F.C. Turner – Photographed myself using Olympus BX50 microscope on the Department of Pathology, University Medical Center Groningen, The Netherlands. License: Public Domain


Definition and Background of Gardnerella Vaginalis

G. vaginalis is a gram-negative, facultative nonmotile anaerobic bacterium, which is the most frequent participant in all vaginitis in women (90% of vulvovaginitis) and causes epidemiological concern in males (urethritis).

Owing to the advanced technologies applied in microbiology, namely, invention of the electronic microscope, and improved laboratory equipment as well as testing methods (isolation and identification of bacteria), the term “nonspecific vaginitis” lost its meaning as the causing reason of the condition was detected and described in detail.

The main way of transmission of the G. vaginalis is sexual infection. Some clinical researches suggest that the rectum may be inhabited with G. vaginalis, hence the bug can travel to genitals from the rectum as well, and thus, self-infection occurs accordingly.

Advanced stages of the infection may lead to severe consequences in the form of pelvic inflammatory disease.

Etiology of Gardnerella Vaginalis

  • Bacterial vaginitis (BV, vulvovaginitis) develops due to the infection with different bugs including B. vaginalis and is a result of their interaction: Lactobacillus, Prevotella; anaerobes: Mobiluncus, Bacteroides, Peptostreptococcus, Fusobacterium, Veillonella, and Eubacterium, Mycoplasma hominis, Ureaplasma urealyticum, Streptococcus viridans, and Atopobium vaginae.
  • Impaired immune response may become a triggering factor for the development of BV.
  • Pregnancy
  • Long-term antibiotic therapy
  • Douching
  • Promiscuity in sexual contacts.

Epidemiology of Gardnerella Vaginalis

BV is very common among women, in the US 29, 8% of the female population was diagnosed with BV with Gram stain in vaginal fluid. Susceptibility increases midst those ones who are nonwhite, practice douching, have numerous sexual partners, have sex with women, smoke. HIV sufferers twice as often get infected with G. vaginalis and develop BV.

Internationally

G. vaginalis is very common worldwide, especially in the countries with humid and hot climate, thus sub-Saharan Africa accounted for over 50% of women infected with G. vaginalis, meanwhile they had a risk of contracting HIV as well.

Presentation of Gardnerella Vaginalis

History

  • Foul vaginal odor (‘’fishy odor”) especially after sexual intercourse (one of the most distinguishing features of BV)
  • Increased amount of vaginal discharges
  • Greyish vaginal discharges
  • Dysuria
  • Vulvar irritation (itchiness).

Physical examination

The labia, introitus, cervix, and cervical discharge are of normal appearance; however, there may be signs of cervicitis. Vaginal walls produce enhanced light reflex without pronounced inflammation. Vaginal mucosa is covered with grey, liquidly homogenous discharges.

Differential Diagnosis of Gardnerella Vaginalis

Candidiasis

This is a vaginal yeast infection caused by Candida albicans (redness and itchiness of vagina; curdlike discharges; cervix: normal findings in speculum examination)

Cervicitis

This describes the condition when there are visible purulent or mucopurulent endocervical discharges in the cervical canal and the cervix easily bleeds on a touch.

Chlamydial genitourinary infections

These are caused by the bacterium called Chlamydia, and is the leading course of infertility in women in the US. Chlamydia affects urethra, salpinges, uterus, nasopharynx, and epididymis, causing unpleasant vaginal discharges, vaginal bleeding between periods, lower abdominal pain, sometimes fever if pelvic organs are involved in inflammation, dyspareunia, and rectal discharges after anal intercourse.

Gonorrhea

The so-called clap is caused by the bacterium Neisseria gonorrhoeae and presents with thin or thick, purulent, and mildly odorous vaginal discharges, sometimes minimal or no discharges, dipareunia, mild lower abdominal pain, bleeding between periods.

Herpes simplex

Herpes simplex is caused by the virus of herpes family  and the signs are ulceration of the penis or vagina followed with insignificant discharges, itchiness of affected zones and there might be enlarged local lymphatic nodes as well as fever and malaise.

Trichomoniasis

This condition causes grey, yellow, or green foul discharges from the vagina urethra, painful urination and intercourse, dysuria, vaginal bleeding after intercourse or unrelated to mensis, genital itchiness and burning sensation.

Table: Differential diagnosis of vaginitis

Clinical Elements Bacterial vaginosis Trichomoniasis Vaginal candidiasis
Symptoms Vaginal odor + +/-
Vaginal discharge Thin, gray, homogenous Green-yellow White, curdlike
Vulvar irritation +/- + +
Dyspareunia +
Signs Vulvar erythema +/-
Bubbles in vaginal fluid + +/-
Strawberry cervix +/-
Microscopy Saline wet mount
Clue cells +
Motile protozoa +
Pseudohyphae +
Whiff test + +/-
pH >4.5 >4.5 < 4.5

Diagnosis of Gardnerella Vaginalis

Laboratory studies

Microscopic examination of the vaginal discharges (clue cells – vaginal epithelial cells with attached bacteria on their surfaces, edges of the cells are stubbed, and “peppered” with coccobacilli; PH is greater than 4,5; color is grey, liquidly and homogenous; there is little amount of polymorphonuclear leukocytes (PMNs)-1 per one vaginal epithelial cell).

Whiff test is positive in 90% of cases (mixing of vaginal fluid with a drop of KOH on a microscope slide detects the presence of amine which is produced by anaerobic bacterial metabolism).

Microscopic evaluation of the bacteria flora is informative while looking for evidence of changes in the overall bacterial predominance, thus normally lactobacilli (large gram-positive rods) is predominant, in BV coccobacilli dominant over any other microflora (anaerobs).

Vaginal cultures is used in order to exclude other pathogenic growth apart from G. vaginalis, namely, Trichomonas species, C. trachomatis, N. gonorrhoeae. G. vaginalis detected in 100% of BV, and in 70% of asymptomatic BV.

Procedures

Colposcopy reflects clean, translucent mucus in external cervical os and opaque vaginal contents in the rear vaginal vault. “Spotted” images visible after Schiller test are pathognomic colposcopic features of Gardnerella vaginalis infection.

Management of Gardnerella Vaginalis

All patients with BV have to be prescribed appropriate treatment based on the results of clinical examinations and cytological tests. Antibacterial therapy has to be taken into account in the first order while pharmacological treatment.

Pharmacotherapy

Medication Duration
Metronidasol 500 mg, orally twice per day 7 days
Metronidasol gel 0,75%, one full applicator intravaginally (5g), once a day 5 days
Clindamycine cream 2% one full applicator intravaginally (5g) at bed time 5 days

Patients taking treatment with metronidazole have to refrain from alcohol during all course and 24 hours afterwords; clindamycin cream softens latex of condoms and diaphragms for 5 days after use.

Surgical intervention is not required in G. vaginalis infection.

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