Table of Contents
- Chemical Properties of Fructose
- Fructose Deposits in Foods
- Fructose Transport inside Cells
- Fructose Degradation in Liver Cells
- Disorders of fructose metabolism
- Essential Fructosemia and Fructosuria
- Hereditary Fructose Intolerance
- Intestinal Fructose Intolerance
- Fructose Biosynthesis from Glycose
- Fructose as a Trigger for Diseases
- Review Questions
Fructose (formerly, levulose for “counterclockwise”) belongs with its molecular formula C6H12O6 to the hexoses, sugars with 6 carbon atoms. Furthermore, it possesses a keto group, which is defined as a carbon atom with a double bond to an oxygen atom. Therefore, fructose is also a ketose. As the molecule is optically active, it exists in two mirror-inverted isomers, called enantiomers. These are usually displayed in the Fischer projection which is an open-chain structure that illustrates the spatial structure of chiral compounds.
The stereo descriptor D stands for dexter (Latin for ‘right’) because of the horizontal high priority rest, i.e. the OH standing lowest in the Fischer projection faces to the right. Conversely, there is the L for laevus (Latin for left). The enantiomer L-fructose, however, is physiologically not significant.
In addition, there exists the Haworth notation where the molecule is drawn as a plain ring. In case of the fructose, this is a special character as it occurs in crystalline form as a six-membered ring (called fructopyranose) and in bound form as a five-membered ring (called fructofuranose). The α- and β-anomers of each ring shape can be converted into each other in an aqueous solution and are in equilibrium with each other.
Fructose Deposits in Foods
Fructose (from Latin,Fructis= fruit) naturally occurs as a monosaccharide, i.e. as a simple sugar, especially in some fruit (for example, 6 g fructose per 100 g apples) and honey (40 g per 100 g). The main part of fructose, however, we absorb as disaccharide sucrose (Latin Saccharum = sugar), our usual industrial sugar. Sucrose consists of α-D-glucose and β-fructose that are linked via a α,β-1,2-glycosidic bond.
Industrially produced foods increasingly contain maize-derived fructose in the form of high-fructose corn syrups. Because of its very sweet flavor and good solubility in water, the syrup is mainly used for sweetening soft drinks.
Fructose Transport inside Cells
In the upper small intestine, sucrose is firstly split into glucose and fructose by the enzyme saccharase. The resorption of fructose from the small intestinal lumen into the enterocytes (Latin for intestinal cells) takes place passively, i.e. energy-independent in contrast to glucose. The fructose transporter GLUT5 (to a lesser degree, also other class II sugar transporters such as GLUT7, 9 and 11) transports fructose along its concentration gradient into the cell interior.
When there is an intra/extra-cellular equilibrium reached, fructose uptake stops and is thus never completely absorbed. This means that in cases of very high dietary intake, fructose becomes osmotically active in the intestine and can cause diarrhea, especially in young children.
It is mainly by means of the GLUT2 transporter that fructose passes through the basolateral membrane of the intestinal cells, i.e. the membrane facing the bloodstream. Through this transport protein, also glucose and galactose are channeled into the blood. Next, the blood flow from the small intestine to the liver occurs via the portal vein.
Fructose Degradation in Liver Cells
Since the fructose uptake by cells is insulin-independent, fructose was used as a sweetener for so-called diet products for diabetics for a long time. Since 2010, however, those products are no longer available.
- In the cells of the liver, the hepatocytes, D-fructose is converted into D-fructose-1-phosphate by the enzyme fructokinase (also called keto-hexokinase) and is thus no longer able to leave the cell. This reaction is ATP-dependent.
- Fructose-1-phosphate is split by aldolase B into dihydroxyacetone phosphate and glyceraldehyde. Dihydroxyacetone phosphate is a byproduct of glycolysis and is further metabolized by Triose Phosphate Isomerase to produce energy.
- In an ATP-dependent reaction, glyceraldehyde is phosphorylated into glyceraldehyde 3-phosphate by the triose kinase and, thus, may enter the glycolysis or the triglyceride synthesis and is deposited as depot fat.
Disorders of fructose metabolism
Following are some pathological conditions associated with fructose :
Essential Fructosemia and Fructosuria
The essential fructosemia and fructosuria are caused by a defect in the fructokinase of the hepatocytes. This enzyme failure leads to the accumulation of fructose in the blood (fructosemia) and urine (fructosuria). It’s a benign and asymptomatic condition (other than fructosuria). This rare disease has no pathological value and is therefore in no need of treatment.
Hereditary Fructose Intolerance
The incidence of these rare, autosomal recessive inherited disease is between 1:20,000 and 1:130,000 depending on the source. The reason is a Hereditary Deficiency of aldolase B, which in hepatocytes normally splits fructose-1-phosphate into dihydroxyacetone phosphate and glyceraldehyde. Aldolase A, an enzyme of glycolysis, is not affected by this defect. The aldolase A, however, metabolizes fructose-1-phosphate 50 times slower than its actual substrate fructose 1,6-bisphosphate.
This results in an accumulation of fructose-1-phosphate in the hepatocytes. This results in a decreased availability of phosphates which inhibits enzymes of the glycolysis and gluconeogenesis, for example, fructose 1,6-bisphosphatase and glycogen phosphorylase. In periods of hunger, life-threatening hypoglycemia occurs because glucose cannot be synthesized from glycogen. Additionally, a hepatomegaly with progressive liver destruction pending liver cirrhosis may develop.
Usually, the first symptoms appear with the start of feeding baby food to the child that contain fruits since breast milk is fructose-free. In mild forms, the child often has an aversion to fruit juices and other fructose-containing foods, prospers badly and cries a lot. In severe cases, the infants are hypoglycemic with symptoms of the adrenergic counter-reaction such as sweating, trembling, vomiting, seizures and even coma. Since the aldolase B is also present in the kidney, the hereditary fructose intolerance also results in kidney damage with proteinuria (the increased excretion of proteins along with the urine)..
Diagnoses are made with molecular genetic tests that cover the 3 most common mutations (A149P, A174D and N334K) of aldose B. One of those three mutations can be detected in 90 % of affected persons. Newborn screenings do not detect the hereditary fructose intolerance.
As is the case with most congenital metabolic disorders, the only effective treatment is complete avoidance of fructose-containing food and sucrose (which is glucose + fructose). For small children, the vitamins contained in fruits and vegetables should be substituted with supplemental products. The partial thromboplastin time (PTT) and antithrombin III are progression parameters as these coagulation parameters respectively factors are already pathologically detectible in case of minor liver damage.
Particular caution should be exercised in case of sorbitol infusions. These are rarely used for reduction of intracranial pressure, due to their osmotic activity. In case of fructose intolerance, sorbitol can also not be degraded and results in the same symptoms including death.
Intestinal Fructose Intolerance
The intestinal fructose intolerance is much more common than the hereditary fructose intolerance. About 1/3 of the people suffer from a fructose malabsorption, which means that only a small amount of fructose is absorbed in the small intestine, so a significant portion passes into the large intestine. Fructose increases the osmotic pressure in the large intestine and, thus, deprives the intestine of liquid.
Furthermore, the bacterial flora of the colon ferments the fructose into carbon dioxide, short-chained fatty acids, and methane. This can lead to diarrhea and meteorism which is why we now speak of an intestinal fructose intolerance. 1/3 of the people with fructose malabsorption suffer from intestinal fructose intolerance.
The causes of fructose malabsorption are diverse: for example, a below-average number or performance of GLUT5 can be the reason but also a rapid intestinal passage of chyme. It is quite possible that fructose intolerance is rather a normal variation than a disease, as is the case with lactose intolerance.
The intestinal fructose intolerance may also develop secondarily based on an inflammatory bowel disease that affects the small intestine. In case of Crohn´s disease, for example, the small intestine’s mucous membrane is damaged so badly that fructose cannot be absorbed anymore. As in the case of hereditary fructose intolerance, the only effective therapy is the avoidance of fructose, sorbitol, and other oligo- (mannitol, xylitol) or disaccharides since those often trigger the same symptoms.
Nevertheless, most persons affected by fructose intolerance tolerate sucrose because of the GLUT2 transport protein. GLUT2 is also localized in the apical membrane of small intestine cells. It is quickly integrated into the cell membrane when the transport protein SGLT1, an ATP-dependent sodium-glucose co-transporter, transports glucose. GLUT2 then transports glucose as well as fructose into the enterocytes, independent from GLUT5. Therefore, it is advisable rather than adhering to a diet without any fruits and fructose-containing vegetables to eat fruits that contain approximately equal proportions of glucose and fructose.
|Fructosemia/ -uria||Hereditary fructose intolerance||Intestinal fructose intolerance|
|Frequency||unknown due to often random diagnoses||1 : 20,000 – 1 : 130,000||about 10 % of the population|
|Symptoms||none||hypoglycemia, liver fibrosis/cirrhosis, kidney damage||meteorism, flatulence, diarrhea|
|Therapy||unnecessary||strict diet without fructose||diet low in fructose|
Fructose Biosynthesis from Glycose
A biosynthesis of fructose from glycose in extrahepatic tissue is possible via the so-called polyol pathway. This particularly takes place in the glandulaevesiculosae, the man´s seminal vesicles, in a testosterone-dependent process. The seminal fluid then has a high content in fructose that serves the sperms contained in the ejaculate as nutrients. The fructose content of fresh ejaculate is determined via the fructolyse test. If the content is decreased, it indicates a hypofunction of testosterone-producing Leydig cells in the testes.
- The aldose reductase initially reduces the aldehyde group at the glucose’s C-atom 1 to a –CH2OH group. The resulting compound is sorbitol.
- Afterwards, sorbitol is oxidized at C-atom 2 by the sorbitol dehydrogenase, and fructose is formed.
NADPH serves as co-substrate for the aldolase reductase and is reduced (oxidized) to NADP. In a second step, NAD+ is reduced to NADH by the sorbitol dehydrogenase. The overall result is the conversion of NADPH to NADH, which is suggested to be responsible for the long-term consequences of a permanently elevated blood sugar level. This is due to the fact that NADPH has an antioxidant effect and is not present in the body as a substrate.
Furthermore, an increased fructose concentration in the eye lens, in case of diabetes mellitus, may lead to the development of cataracts via the polyol pathway. Sorbitol and glucose are highly osmotic and cause swelling of the eye lens.
Fructose as a Trigger for Diseases
Several studies, including a study by the German Institute of Human Nutrition, prove a correlation between increased fructose uptake and obesity. Fructose does not only increase lipogenesis but also leads to an increased feeling of hunger since no insulin is released that also is a saturation hormone.
In addition, a fructose-rich diet leads to an increase in plasma lipids and insulin resistance. Thus, fructose acts permanently diabetogenic and to a higher degree than glucose. All in all, fructose increases the incidence of metabolic syndrome, which is defined as the joint appearance of obesity, dyslipidemia, impaired glucose tolerance and hypertension.
Further studies show a correlation between fructose uptake and non-alcoholic fatty liver (steatosis hepatis) which is a preliminary stage of liver cirrhosis.
Also, the risk of developing gout is increased dramatically, particularly through fructose-sweetened soft drinks as evidenced by prospective studies including thousands of patients. The reason is the consumption of ATP during fructose degradation. IMP accumulates and leads to an increase of uric acid concentration via purine metabolism.
According to recent studies, the risk of cancers such as the pancreatic carcinoma increases through a fructose-rich diet. Fructose contributes to the synthesis of nucleic acids which encourages the proliferation of cancer cells.
For these reasons, the Food and Agriculture Organization of the United States recommends reducing the daily intake of free sugars such as fructose to less than 10 % of the total amount of energy.
Solutions can be found below the references.
1. Fructose absorption by small intestine cells takes place…
- …via sodium symport.
- …insulin-dependent via the transport protein GLUT2.
- …along the concentration gradient via the transport protein GLUT5.
- …energy-dependent via a sodium-potassium ATPase.
- …only in the form of sucrose via the transport protein GLUT2.
2. A reduced output level of fructose in the ejaculate detected by fructolyse test implies a malfunction of which cells?
- Leydig cells
- Sertoli cells
- Prostate cells
- Epididymis cells
3. What does not apply to the hereditary fructose intolerance?
- It is an autosomal recessive disorder.
- It is caused by a defect of aldolase B.
- In periods of fasting, life-threatening hypoglycemia can occur.
- Children should not be breast-fed because galactose leads to the same symptoms.
- For affected persons, a hepatomegaly may subsist at the time of diagnosis.