Table of Contents
- Etiology of Bovine Spongiform Encephalopathy
- Transmission to Humans
- Signs and Symptoms of Bovine Spongiform Encephalopathy
- Diagnosis of Bovine Spongiform Encephalopathy
- Pathogenesis of Bovine Spongiform Encephalopathy
- Prevention of Bovine Spongiform Encephalopathy
- Diagnostic Criteria of CJD
- Treatment of Bovine Spongiform Encephalopathy
Etiology of Bovine Spongiform Encephalopathy
The postulated cause of the spread of mad cow disease is MBM (meat and bone meal), which is a concentrated, protein-rich supplement feed given to the dairy cows. Due to this reason, dairy cows are more prone to develop the disease as compared to beef cattle.
The infectious agent, which is the prion protein, can survive in temperatures of more than 600 degrees Celsius. Some theories also state that the disease is caused by a virus. The animals carry an allele which converts the normal alpha helical arrangement of the protein into beta pleated sheets. This leads to the formation and accumulation of plaques causing degeneration and holes in the brain, leading to mental impairment of the animal.
Healthy animals can become infected by coming in contact with infected animals. Two leading hypotheses suggest that:
- The disease might have come from other species, presumably sheep, or
- It may have happened before as well, in the past centuries.
The incubation period varies from 2.5 to 5 years with the peak age of onset of 5 years.
Transmission to Humans
In humans, the disease variant is known as Creutzfeldt-Jakob disease (vCJD or nvCJD). It is said that the infectious agent is found in the brain and spinal cord, but it is also present in every tissue of the body of the infected cattle. The disease is likely to occur if a person consumes food that has been contaminated with the brain and spinal cord of the infected animal.
The disease is not curable and leads to a fatal end.
The classical clinical features of CJD include:
- Rapid cognitive decline,
- Myoclonus and the development of akinetic mute state.
- The neurological symptoms need to be progressive in nature.
The diagnosis of the disease can be done by the following methods:
- Electroencephalography demonstrates sharp wave pattern.
- Cerebrospinal fluid analysis shows misfolded proteins.
- Magnetic resonance imaging of the brain shows damage in the caudate nucleus and putamen.
- Neuron specific enolase: this tumor marker is mostly raised in patients with Creutzfeldt-Jakob disease.
Signs and Symptoms of Bovine Spongiform Encephalopathy
As the disease has a very long incubation period, its symptoms and signs occur very late. The infected animal usually has an abnormal gait, and when muscles are affected, the animal loses the ability to stand. The animal might exhibit changes in behavior, anxiety, aggression, persistent rubbing or teeth grinding due to pain. The disease eventually leads to coma and death.
Diagnosis of Bovine Spongiform Encephalopathy
It is very difficult to diagnose the disease at an early stage due to its very long incubation period of months to years. So, presumptive diagnosis can only be made by the developing signs and symptoms. At the time of development of signs and symptoms, however, the disease has virtually progressed into every body tissue and fluid.
Definitive diagnosis can only be made by examining the postmortem brain and body tissues by using neuropathological and immune-histochemical stains. The toxic prion protein is also present in the blood and urine of the infected animal, but the amount of the protein is very low. Thus, scientists are still working on developing methods to diagnose the disease via the blood and urine.
Pathogenesis of Bovine Spongiform Encephalopathy
Pathogenesis of bovine encephalopathy is still not well understood, and research is still under process. Although the disease affects the brain and spinal cord, pathogenesis occurs in other body parts as well.
One theory strongly suggests that there is a reason why the presence of the infectious prion protein was demonstrable in the macrophages of the peyer’s patches of the ileum. Months after inoculation, the protein was only found in the peyer’s patches which led the researches to think that the organism only replicates there.
Prevention of Bovine Spongiform Encephalopathy
Prevention can only be done by regulating the constituents of the feed given to dairy cows. In the UK and US, body parts like brain, spinal cord and eyes are considered to be high risk and proper methods are proposed for their disposal.
Diagnostic Criteria of CJD
The most important magnetic resonance imaging findings in sporadic CJD are high-intensity signals in the caudate, putamen, or cortex on diffusion-weighted imaging and FLAIR sequences.
In variant CJD, symmetrical hyper-intensity of the posterior thalamus relative to the anterior putamen on T2 or FLAIR MRI images is the most common finding.
The most important finding on EEG in patients with CJD is periodic, triphasic sharp wave complexes.
The European Union has adapted new diagnostic criteria for the pre-mortem diagnosis of sporadic and variant CJD in 2017:
Progressive neurological syndrome and CJD confirmed via neuropathological, immunohistochemical, or biochemical testing.
Rapidly progressive cognitive impairment plus two of the following (myoclonus, visual or cerebellar problems, pyramidal or extrapyramidal features, akinetic mutism) plus generalized periodic sharp-wave complexes on EEG.
Rapidly progressive cognitive impairment plus two of the following (myoclonus, visual or cerebellar problems, pyramidal or extrapyramidal features, akinetic mutism) plus the typical MRI findings described above.
Rapidly progressive cognitive impairment plus two of the following (myoclonus, visual or cerebellar problems, pyramidal or extrapyramidal features, akinetic mutism) plus positive cerebrospinal fluid results.
Progressive neurological syndrome and positive real-time quaking-induced conversion in CSF or other tissues.
Rapidly progressive cognitive impairment plus two of the following (myoclonus, visual or cerebellar problems, pyramidal or extrapyramidal features, akinetic mutism) for a duration less than 2 years.
Treatment of Bovine Spongiform Encephalopathy
There is no treatment of mad cow disease up to date.