Biotechnology: Recombinant DNA & Gene Amplification

by Georgina Cornwall, PhD

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    Hello and welcome back. The next two lectures in this series will be about biotechnology. We will begin by looking at the basics with recombinant DNA and gene amplification. By the end of this lecture, you should be able to explain how restriction enzymes worked to produce recombinant DNA as well as discussed the role of gel electrophoresis in many biotechnologies. And then you will be able to explain how vectors are used in molecular cloning as well as describing the process of polymerase chain reaction in DNA amplification. Let us begin by taking a look at what recombinant DNA is. Just the basics. First of all, we could have for example a human cell and a bacterial cell. Recall that a bacterial cell has not only a long circular chromosome that you see here, but also has the potential to take up plasmids or produce plasmids, which are much smaller circular pieces of DNA. The plasmid will be sort of emphasized in these next lectures because that is an important part in passing on genetic information between bacteria. Either way, we can take a gene from a human cell using restriction enzymes which cut DNA and we can cut open the bacterial chromosome or plasmid and we can add the human DNA or another species DNA into that bacterial chromosome or plasmid and will have a recombinant piece of DNA. This DNA, in particular, consists of the human growth hormone for example. Now we need to put this piece of DNA somewhere so that perhaps we can get it into a eukaryotic organism. We put that DNA into a bacterial cell or a viral cell, any other host type cell and we now have a bacterial cell that contains recombinant DNA and we can use a number of different...

    About the Lecture

    The lecture Biotechnology: Recombinant DNA & Gene Amplification by Georgina Cornwall, PhD is from the course Biotechnology. It contains the following chapters:

    • Recombiant DNA
    • Cutting the DNA
    • Gel Electrophoresis
    • Bacterial Transformation
    • Molecular Cloning
    • Possible Host-Vector Systems
    • Transforming Eukaryotic Cells
    • The CRSPR/Cas9 System
    • The Polymerase Chain Reaction

    Author of lecture Biotechnology: Recombinant DNA & Gene Amplification

     Georgina Cornwall, PhD

    Georgina Cornwall, PhD

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    By fatma a. on 30. January 2017 for Biotechnology: Recombinant DNA & Gene Amplification

    it was useful but it did not go into detailed on the variation or different types of PCR which i was looking for. like real-time PCR, allel specific pcr, nested pcr. where can I get these information from?

    The essentials, well selected and concisely explained
    By Eduard F. on 22. December 2016 for Biotechnology: Recombinant DNA & Gene Amplification

    Well balanced in providing key information while dedicating enough time to explanations and background comments