Biotechnology: DNA Libraries, Analysis & Applications

by Georgina Cornwall, PhD

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    Welcome back to our second lecture on biotechnology. In this lecture, we will apply some of the techniques that we learned in the first lecture and explore how DNA libraries are put together as well as examine some DNA analysis techniques. And finally we will look at some applications of biotechnology in medicine and agriculture. So by the end of this lecture, you should be able to explain the benefit of DNA libraries as well as compare RFLP and STR in DNA fingerprinting. What are those you may ask? Well stay with me and you will find out. By the end of this lecture, you will also be able to discuss some of the applications of biotechnology in both agriculture and medicine. Let us move into the beginning of genomic libraries. Genomic libraries are similar to our libraries except that there are volumes of books that have overlapping sequences. They are not independent volumes. There is often some repeats in a DNA library. They contain a representation of the entire genome of an organism. The whole point is to store that genome and fragments so that we can maybe sequence it or figure out some of the genes in the genome too. When we think about storing these fragments in a library essentially all we are doing is taking DNA fragments from an organism's DNA workshopping them with restriction enzymes. And then we will insert them into a plasmid vector. We already know how that works from the previous lecture and then we take those vectors and ask bacteria to transform and pick up these vectors and store them for us in a nice neat library. Each piece of DNA is in a different bacteria and there are perhaps hundreds of bacteria to represent one genome of maybe the fruit...

    About the Lecture

    The lecture Biotechnology: DNA Libraries, Analysis & Applications by Georgina Cornwall, PhD is from the course Biotechnology. It contains the following chapters:

    • DNA Libraries
    • Probes and Blotting Methods
    • DNA Fingerprinting
    • Short Tandem Repeats
    • Biotechnology in Medicine
    • Biotechnology in Agriculture
    • Transgenic Animals

    Author of lecture Biotechnology: DNA Libraries, Analysis & Applications

     Georgina Cornwall, PhD

    Georgina Cornwall, PhD

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