Lectures

Preparation of Antibodies

by Peter Delves, PhD
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    About the Lecture

    The lecture Preparation of Antibodies by Peter Delves, PhD is from the course Immunodiagnostics. It contains the following chapters:

    • Production of Polyclonal Antibodies
    • Hybridoma Production of Monoclonal Antibody
    • Phage Display Production of Monoclonal Antibody

    Included Quiz Questions

    1. Myeloma cell line
    2. Melanoma cell line
    3. Bacteriophages
    4. B-cell stimulating cytokines
    5. Fusion of normal T-cells
    1. B cell antigen specificity and myeloma cell immortality
    2. B cell antigen specificity and myeloma cell splenic specificity
    3. B cell splenic specificity and myeloma cell immortality
    4. B cell immortality and myeloma cell splenic specificity
    5. B cell immortality and myeloma cell antigen specificity
    1. IGHV and IGLV
    2. mRNA
    3. Flexible linker
    4. Tumor antigen
    5. Fused myeloma and B cells
    1. Antibody on cell surface and gene for encoding that antibody within cell
    2. Identical antibodies on cell surfaces
    3. Two IGHV segments linked with a flexible linker
    4. mRNA expression for various antibodies
    5. Specific primers that amplify cDNA

    Author of lecture Preparation of Antibodies

     Peter Delves, PhD

    Peter Delves, PhD


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