Paroxysmal Nocturnal Hemoglobinuria (PNH): Clinical Pathology

by Carlo Raj, MD

My Notes
  • Required.
Save Cancel
    Learning Material 2
    • PDF
      Slides Paroxysmal Nocturnal Hemoglobinuria PNH.pdf
    • PDF
      Download Lecture Overview
    Report mistake

    00:01 Further, well, episodic, that’s your paroxysmal.

    00:04 What’s the second, second time that you can develop this acidotic environment? Respiratory acidosis at night.

    00:13 Also, when would you have lactic acidosis? When you exercise, at some point, you fell cramps.

    00:18 Well, I feel cramps all the time.

    00:20 That’s because I’m out of shape.

    00:22 And so when I exercise, I end up going through what? The skeletal muscle undergoes anaerobic glycolysis.

    00:29 When you have anaerobic glycolysis, you’re producing more lactic acid.

    00:33 So what kind of acidosis is this? Metabolic acidosis.

    00:37 So take a look at the two bullet points here.

    00:40 Both of these are acidotic environments.

    00:43 Which means what? If a patient has PNH and is exposed to these acidotic environments, then guess what happens? Your RBCs will then become destroyed episodically.

    00:55 Not all the time.

    00:57 Remember I told you earlier the term episodic in pathology.

    00:59 It is very important because it will help you distinguish one type of pathology from another, one that is continuous versus one that’s episodic.

    01:10 Overtime, earlier I told you when you’re losing hemoglobin, and you tell me about where iron is bound to, it’s bound to the heme component, right? So if you’re urinating your hemoglobin along with it, there goes my iron.

    01:25 So also look for microcytic anemia, iron deficiency.

    01:28 Diagnosis, you’re going to use a flow cytometry.

    01:31 And with this, ladies and gentlemen, I’m going to walk you through more detail that’s necessary in the next slide, but at this point, flow becomes very important for you and you’re going to be looking for this glycosylphosphatidylinositol proteins, those GPI-linked protein.

    About the Lecture

    The lecture Paroxysmal Nocturnal Hemoglobinuria (PNH): Clinical Pathology by Carlo Raj, MD is from the course Hemolytic Anemia – Red Blood Cell Pathology (RBC).

    Included Quiz Questions

    1. Lactic acidosis
    2. Respiratory alkalosis
    3. Sedentary lifestyle
    4. Travel to high altitudes
    5. Metabolic alkalosis
    1. Flow cytometry
    2. Serotonin release assay
    3. Acidified serum lysis test
    4. Osmotic fragility test
    5. Metabisulfite screen

    Author of lecture Paroxysmal Nocturnal Hemoglobinuria (PNH): Clinical Pathology

     Carlo Raj, MD

    Carlo Raj, MD

    Customer reviews

    5,0 of 5 stars
    5 Stars
    4 Stars
    3 Stars
    2 Stars
    1  Star