00:01
So enzymes being the key players in this game of metabolism
can be activated or inhibited.
00:09
When we consider them being inhibited, they can either be
competitively inhibited or allosterically inhibited.
00:17
Now you might understand competition but what on earth is allosteric.
00:22
Competitive inhibition, we'll take a look at first,
involves our enzyme and recall that each enzyme has an active site.
00:30
And generally the substrate would bind into the active site.
However, on occasion, something else gets there first,
this would be an inhibitor molecule or a competitive inhibitor
because it is competitively binding to the site.
00:46
We have a lot of drugs and productions these days
that are competitive inhibitors.
00:52
For example SSRIs, selective serotonin reuptake inhibitors actually
binds to receptors that should be reuptaking serotonin
and prevent the binding of serotonin itself
so it cannot be reuptake and it spends more time
in the synaptic cleft and makes us happier.
01:12
It's in a lot of antidepressents drugs.
01:15
Anyway, moving forward from there to the allosteric thing.
What on earth is an allosteric site?
Well, it's pretty simple. Anything other than the active site
that's a binding site on an enzyme.
01:27
So anything other than the site that takes a substrate
is called an allosteric site.
01:33
So allosteric sites could bind another molecule.
We'll either see allosteric activation or allosteric inhibition.
01:43
In this example, we'll look at inhibition.
We have an allosteric inhibitor binds to the allosteric site
changes a normal shape active site, an active active site,
into an inactive active site
and so the substrate that should fit into
the example of the middle no longer can fit
and so the enzyme activity has been inhibited or shut down.
02:10
The opposite can happen with allosteric activation. We have an enzyme.
It has an active site that is in not the ultimate form,
and let's say we want to turn it on.
02:20
We can now have an allosteric activator binds somewhere else
on the enzyme, to the allosteric site, mystery there,
and it changes the shape of the receptor site, the active site,
so that the substrate can bind into that active site
and the enzyme can go ahead and do the job that it's supposed to do.
02:40
So allosteric inhibition and activation,
both involve a site somewhere else on the enzyme
that changes the confirmational shape of the active site.