Lectures

Tissue Typing, Immunosuppressive Drugs and GVHD

by Peter Delves, PhD
(1)

Questions about the lecture
My Notes
  • Required.
Save Cancel
    Learning Material 2
    • PDF
      Slides Transplantation Immunology.pdf
    • PDF
      Download Lecture Overview
    Report mistake
    Transcript
    This need to match the MHC between the donor and the recipient necessitates tissue typing of the donor and the recipient. This patient has developed renal failure and needs a kidney transplant. He has been typed for HLA-A, HLA-B and HLA-DR. And as we can see, this particular individual is HLA-A2, A5, B7, B13 and DR2, DR1. Here we have a number of different patients that are awaiting a transplant. They are placed on a register with many others. Each of whom are likely to have different HLA types as shown on this slide. When tissue typing occurs, a potential donor is HLA typed and each kidney is sent to a hospital where a good HLA match patient is waiting to have the graft transplanted. A blood sample from the donor is also sent to the hospital. In the cross match, donor B-cells are mixed with recipient’s serum. In this case, preformed antibodies are binding the donor cells. This transplant cannot take place. However here we see that the recipient has no antibodies against donor cells and the transplant can go ahead. Preformed anti-donor antibodies can result from pregnancy, blood transfusion, or indeed a previous transplant. Let’s look at the mechanisms of action of immunosuppressive drugs. These are almost always required, because it’s almost impossible to get an identical match between the donor and the recipient unless there is an identical twin. There is almost always some mismatch as regards the MHC. Here we can see an anti T-cell receptor antibody that is used to block the interaction between the T-cell receptor and the MHC. The drugs, cyclosporine and FK506 inhibit the calcineurin signaling pathway. CTLA4-Ig interferes with the co-stimulatory interaction between B7 and CD28, thereby preventing co-stimulation and activation of T-cells. Both of these approaches, using cyclosporine, using FK506,...

    About the Lecture

    The lecture Tissue Typing, Immunosuppressive Drugs and GVHD by Peter Delves, PhD is from the course Transplantation Immunology. It contains the following chapters:

    • Tissue Typing
    • Mechanisms of Action of Immunosuppressive Drugs
    • Graft versus Host Reaction

    Included Quiz Questions

    1. Rapamycin
    2. Cyclosporine
    3. FK506
    4. OKT3
    5. CTLA4-Ig
    1. A recipient with a good HLA match can have pre-formed antibodies which will reject the transplant
    2. A recipient with some antibodies towards a donor's serum can still receive transplanted tissue
    3. Tissue transplant is based on highest need, not highest HLA match
    4. A good HLA match is considered one or two HLA matches
    5. A recipient with a previous transplant is not at greater risk for tissue rejection
    1. Cytotoxicity of donor cells due to recipient response
    2. Hematopoietic stem cell transplant
    3. Recipient immunosuppression
    4. Donor T cells recognizing recipient MHC as foreign
    5. Destruction of recipient cells by donor cells

    Author of lecture Tissue Typing, Immunosuppressive Drugs and GVHD

     Peter Delves, PhD

    Peter Delves, PhD


    Customer reviews

    (1)
    5,0 of 5 stars
    5 Stars
    5
    4 Stars
    0
    3 Stars
    0
    2 Stars
    0
    1  Star
    0