00:01
Now we have a few more mechanisms to cover
in levels of possibility for regulating gene
expression. RNA interference, we have already
visited alternative splicing as well as RNA
editing.
00:16
So what is RNA interference? RNA interference involves
small interfering RNAs or siRNAs and micro RNAs (miRNAs).
00:27
So we introduced those when we looked at translation.
Lots of different RNAs involved in the
process of gene expression. Here is
where these guys come into play. I told you
I will bring them up again. They can be involved
in selected degradation of messenger RNA.
00:48
They will breakdown that messenger RNA perhaps
by binding with different parts of it to make
them inaccessible to the ribosomes. We could
have inhibition of translation because they
again get in the way may be block sites for
binding of TRNAs. And then we could have alteration
of chromatin structure. In the section on
epigenetics, I also introduced the idea of
RNAs becoming associated with chromatin and
impacting how enzymes can access it. All sorts
of different ways that we can regulate expression
even after we have RNA produced through the
process of transcription. Lots of things, alternative
splicing. Recall that there are different
patterns that we could remove introns and
exons. We can have different polypeptides
from the same gene because we take a different
selection of expressed sequences from the
original RNA and slice them alternatively
so that we get different protein results.
02:06
Gene expression is regulated there naturally.
Then we have RNA editing something we have
not previously discussed, but RNA editing
is where there are enzymes out in the
cytosol that can potentially alter the RNA
transcripts so that it doesn't produce the
same proteins. Again you could get multiple
different peptides from one gene because there
are other molecules out there that are interested
in editing. We are learning more and more
about these. One example that we are fairly
well aware of is apolipoprotein B, which is
involved in the formation of LDLs, low-density
lipoproteins that carry cholesterol around.
02:53
There are two forms of this apolipoprotein B.
There is one that is much longer and one
that has been cut a section of the messenger
RNA, it turns out has been cut out after processing.
03:08
So out in the cytosol, section gets cut off and
we see that the edited form only appears in
the intestinal cells, so there has to be some
reason that the edited form is more useful
there in packing LDLs differently than we
see in the liver where we see the full form
in packing LDLs. RNA editing, a big
upcoming field. Again,
another way we see RNA editing in conjunction
with alternative splicing is in serotonin
receptors. There are loads of serotonin receptors
and some of them are the way they are because
of RNA editing and some because of alternative
splicing. But the point is having many different
sorts of serotonin receptors clearly we might
be able to explain some of these varieties
of receptors impact how people deal with
serotonin and could eventually be associated
with different behaviours and effectiveness of drugs and
so on and so forth. Anyway, so really really interesting
fields in gene expression at the moment and
lots and lots of things to learn about. There
is more and more coming out every day.