RAS Protein & Signal Transduction – Carcinogenesis

by Carlo Raj, MD

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    Focus on RAS. You will find this to be quite interesting. The way that I will approach this, I'm going to quickly walk you through the verbiage. And then I will walk you through the illustration. And it is up to you to make sure that you put the two concepts together. However you wish to learn. To begin with I will tell you couple things that are important. RAS is associated with G protein. Tell me about the G protein from biochemistry. Alpha, beta, gamma, right. Alpha, beta, gamma, when associated with G protein would mean that it's inactive. What do you want to do to activate G protein. You get rid of the beta and gamma, you hang on to the alpha. Alpha, active. The specific activity of RAS and how it works is going to be through GTP. So activated GTP binding then deactivated by normally inherent GTPase activity. Stop there. That is a huge point. GTP is active. GDP is inactive. What do you need to then create active? Phosphorylate. And it's called guanine triphosphate. Once the job of RAS has been completed. Then you are going to inactivate the RAS. How do you inactivate the RAS? By inactivating the G protein. So now the GTP will be acted upon by GTPase. Same concept as ATP and ATPase. What does ATPase create? ADP. What does GTPase create? GDP. What's that mean to you? Inactive. This is normal. So, what happens in cancer? You never want to stop RAS activity. Which means that RAS has to be bound to whom eternally? GTP. What enzyme might you want to inhibit so that you can guarantee that GTP is bound to RAS. GTPase. So on your boards, or in general, if you're going to have cancer that GTPase activity...

    About the Lecture

    The lecture RAS Protein & Signal Transduction – Carcinogenesis by Carlo Raj, MD is from the course Cellular Pathology: Basic Principles.

    Included Quiz Questions

    1. Low GDP, high GTP
    2. Decreased RAS activity
    3. Increased GTPase activity
    4. Low GTP, high GDP
    5. G-proteins coupled to beta and gamma particles
    1. Increased quiescence of the cell
    2. Decreased or lost GTPase activity
    3. Decreased GAP activity
    4. Increased activation of the cell
    5. Increased G-protein cascade activation
    1. Activation of transcription in the nucleus
    2. Increased GDP
    3. Upregulation of RAF-1
    4. Decreased GTP
    5. Activation of growth factor receptors
    1. RAS would not be anchored in the membrane
    2. Growth factor would fail to bind to receptor
    3. MAP kinase pathway would not be able to enter the nucleus
    4. GDP would not be transformed back to GTP
    5. Beta and gamma particles would not be able to disassociated from the G protein complex
    1. 25-40%
    2. 5-10%
    3. 15-20%
    4. 60-75%
    5. 100%

    Author of lecture RAS Protein & Signal Transduction – Carcinogenesis

     Carlo Raj, MD

    Carlo Raj, MD

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