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Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology

by Kevin Ahern, PhD
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    About the Lecture

    The lecture Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology by Kevin Ahern, PhD is from the course Analytical Techniques in Biotechnology.


    Included Quiz Questions

    1. Each spot on the final gel corresponds to a single protein
    2. The first step involves separating proteins by molecular weight
    3. SDS is necessary to separate proteins by pI
    4. The smallest proteins are found at the top of the gel
    1. The charge or pI value
    2. The mass of the protein
    3. The molecular weight of the protein
    4. The size of the protein
    5. The shape of the protein
    1. It helps in the neutralization of all the charge present on each protein molecule under investigation
    2. It helps in the protecting the negatively charged amino acids on the proteins from the attack of cations present in the electrophoretic buffer
    3. It helps in the protecting the positively charged amino acids on the proteins from the attack of anions present in the electrophoretic buffer
    4. It provides a charge to the neutral proteins so that these can pass easily through the gel column during electrophoresis
    5. It protects the proteins from degradation and activity loss stimulated under the effect of electric current

    Author of lecture Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology

     Kevin Ahern, PhD

    Kevin Ahern, PhD


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