00:01
Let's remodel this scar.
00:04
So those of you who may have had
a surgical procedure,
know that very early on
of the procedure
is kind of a red looking,
angry thing.
00:15
And over time, that scar gets
smaller and smaller, and more white.
00:21
Why does that happen?
So again, we're looking at
overlapping effects.
00:27
And slightly different
than what we've seen previously,
but inflammation,
acute and chronic,
kind of occurs over a period of
two to seven days after injury.
00:36
Granulation tissue overlapping
five to 10 days.
00:40
And it kind of peaks
in that period of time.
00:43
And then the dotted line
that's going up,
that's collagen accumulation.
00:49
And there will be remodeling.
00:51
So the cells that are in there,
that are making the matrix
are actually as it turns out,
modified smooth muscle cells.
01:00
They are fibroblast like,
but smooth muscle like.
01:03
And over time, they can contract,
and we get a smaller wound.
01:09
And over time, we also release
matrix metalloproteinases,
and don't release tissue inhibitors
and metalloproteinases.
01:18
And we get some
restructuring of that matrix.
01:22
And over a period
of weeks to months,
that wound will contract.
01:27
This has important ramifications
not only in terms of making
that scar on your abdomen
smaller and less conspicuous,
but let's say, I have a total burn
on my entire hand, and it's scars.
01:39
And then if I don't have
regular physical therapy,
that wound will contract.
01:45
And instead of having a hand,
I'll have kind of a claw.
01:49
So wound contraction actually has
ramifications.
01:52
As we'll see,
when we talk about
the complications
associated with wound healing.
02:02
Then this is now wound healing
kind of one on one.
02:06
This is what we worry about
a kind of the surgical level.
02:11
So surgeons talk about
healing by first intention
and healing by
secondary intention.
02:18
Healing by first intention
or by primary intent.
02:21
We have a nice kind of
circumscribed injury,
such as an incision
that we cut into the tissue,
and then we sew back together.
02:31
So we approximate the edges.
02:33
Clean, not infected, all of that.
02:36
But the injury
that's going on there
and then typical
wound healing process
will lead us to have
all the usual things.
02:44
We will get inflammatory
mediators to get elaborated,
we'll get a little bit
of angiogenesis,
we will grow into that area.
02:52
So over time,
we'll get granulation tissue
right on schedule,
seven to 10 days.
02:56
And then in that area of injury,
we will regenerate mostly,
but we will lay down
a little bit of scar.
03:03
Okay, that's healing
by first intent,
where we don't have
big areas of damage.
03:09
And it's just following
the usual processes
that we talked about.
03:12
And then there's healing
by second intent.
03:15
And this happens when we don't want
to necessarily close it up.
03:19
This will actually happen
quite frequently.
03:22
You'll see it on the wards
when a patient has a large
decubitus ulcer, for example.
03:27
Where the tissue has not healed,
and there's a big area
of ulceration in the skin.
03:31
You don't want to close that up
because there will be bacteria
and other things in there
that will get infected.
03:37
So what you do is every day,
as a medical student,
go in,
pack the wound with sterile gauze,
sometimes containing antibiotics.
03:45
And then the next day,
you come along,
take out the gauze
and debris the wound
and you'll see
granulation tissue in there,
and slowly but surely,
that big ulcer
will eventually close in
and you'll get skin
over the surface of that.
03:59
That's healing by second intent.
And we do it all the time.
04:02
But it's a much bigger process
with much more inflammation,
much more injury,
and greater angiogenesis,
and we count on eventually
that getting smaller,
and smaller, and smaller
because of wound contraction.
04:14
So, here we have this big open area
of ulceration, decubitus ulcer
with a big scab in it,
which is just
coagulated blood products.
04:25
And we are now recruiting
because of the injury,
all the macrophages that are
going to drive angiogenesis
as you see them
in the new capillaries.
04:33
And you're going to begin
the process of filling this in
slowly but surely.
04:38
And over time, you will have
a big area of fibrosis
that will eventually
over time contract.
04:44
Note to, on this last piece.
04:48
See in the middle one where
there was a new capillary sprout,
that was necessary to maintain
this highly metabolic scar activity?
Once we have scar,
scar is actually kind of low
metabolic activity.
05:00
It doesn't take much blood supply
to maintain a scar
because it's mostly
extracellular matrix.
05:07
So over time, not only does that
wound contract and gets smaller
because of the myofibroblast,
but it also blanches.
05:16
We don't need as much vasculature,
so that remodels and that goes away.
05:20
And that's why a wound over a longer
period of time starts out very pink
because of neovascularization,
and it ends up very white
because we don't need
much blood vessel supply
to keep that scar alive.
05:31
So, long term scars,
get wider and wider,
paler and paler.
05:37
And they get smaller and smaller,
up to a point.
05:41
Primary versus secondary healing.
05:43
So primary healing,
there's mild inflammatory infiltrate
because there's not much damage.
05:47
In secondary healing,
healing by secondary intent,
more intense inflammation
because we have these big
areas of injury.
05:55
There is less granulation tissue,
in the primary intent.
05:58
There is greater volume
of granulation tissue
and therefore a greater amount
of resulting scar.
06:04
And in the primary wound,
there is not much
significant wound contraction.
06:08
You really haven't had
much damage,
but there may be significant
wound contraction that occurs
in healing by secondary intent.
06:17
So with that, we've kind of walked
all the way through,
from acute to chronic inflammation,
to angiogenesis, to wound healing,
and we are ready to talk about
the complications of wound healing.