00:01
So how do we diagnose
Kala-azar or other
leishmaniasis infections?
One way, that's very well documented
is by polymerase chain reaction.
00:11
We look for the
DNA of the organism
in samples from either
a lesion or from blood,
depending on the
kind of disease.
00:19
And you can learn more about PCR
in our earlier
lectures on this topic.
00:24
We can also examine histology
material from biopsies.
00:29
You can take biopsies
of lesions for example
and look in the microscope and see
the typical forms of the parasite.
00:36
There is also an antibody test,
which is in ELISA-based test
which can be done in the field.
00:42
This is a very easy
to carry out test,
which involves a microscope slide
that has been coated with a matrix.
00:50
And that matrix at one end contains
antibodies that are looking
for either proteins or proteins that
can be used to detect antibodies.
01:01
In this case we're asking, does the
person have antibodies against leishmania?
So what you do in practice is
you take a little bit of blood,
a drop of blood will do,
and you drop it at
one end of the slide.
01:14
The blood then begins to move
across the matrix on the slide
and after a certain amount of
incubation, 10 or 15 minutes,
you can then read out the
result as either negative
or positive, or invalid.
01:31
So embedded in this slide
is a compound that will react
with every blood sample,
it is something known to be in all
blood, infected or uninfected,
so that gives you the line that
tells you the assay worked.
01:44
And then the second slide there that's
label positive, there's an additional line
which would tell us in this
case, that the sample contains
antibodies to leishmania,
because on that line on the side,
we've embedded antigens
against the parasite.
01:58
So a very rapid inexpensive field
applicable test for diagnosis.
02:04
We can treat
leishmania infections
with a variety compounds, one of them
is Sodium stibogluconate, shown here.
02:13
And another is Amphotericin B.
02:16
Unfortunately, there is growing resistance
to the use of some of these compounds
necessitating other
approaches to treatment
and one of those is
Cycloguanil pamoate.
02:28
A number of vaccines are
under development because
leishmania particularly the visceral
form is extensive and lethal,
many vaccines are
being developed
and eventually one of them
or more will come to market.
02:41
Under exploration,
our whole parasite vaccines
where we grow the parasites
they are in activated,
so they're no longer infectious,
they can be injected.
02:50
Individual proteins purified
from the parasite or
recombinant proteins
made by recombinant
DNA technology.
02:59
Synthetic peptides,
short amino acid sequences,
20 to 40 amino acids
that are synthesize,
which can be injected to give
rise to immunological protection.
03:09
And finally, DNA vaccines
in which plasmids containing
coding regions for various leishmania
protein can be injected into the host.
03:18
Interestingly,
another type of vaccine that's recently
shown some promise
in animal trials
is a vaccine against sand
fly salivary proteins.
03:30
Apparently,
these are required for leishmania
to be able to establish
itself in the host.
03:36
So if you can make antibodies
against the fly proteins,
this could be protective.
03:41
So an interesting approach
to dealing with this disease.
03:45
How do we prevent
leishmania infection?
Well, we can use insecticides to
try and get rid of the sand fly.
03:51
You could spray
your entire home,
you could just use bed nets,
and spray those as well.
03:58
And of course, screens and curtains
would help as well as that.