00:00
Well, let us have a look at the neuromuscular
junction. The neuromuscular junction consists
of an axon that passes down from the nerve,
the nerve fibre, coming down from the spinal
cord reaches the muscle. It then branches
into a number of different sorts of branches,
maybe two, three maybe many. And then those
axons interact with individual skeletal muscle
fibres through the neuromuscular junction.
00:37
Sometimes it is called a motor endplate. And
what happens then, is that the wave of depolarization
passing down or the action potential passing
down through the axon, then releases neurotransmitter
from the synaptic cleft. And the synaptic cleft
and the muscle have very specialized structures,
some of which you can see here. Maybe not
so clearly, but let me just explain to you
what they are. The axon terminal has lots
and lots of mitochondria there. That energy
is required to retake in or reabsorb back neurotransmitter
and has also lots and lots of vesicles there,
synaptic vesicles. These synaptic vesicles
contain the neurotransmitter substance and
when the axon potential passes down to this
terminal, those synaptic vesicles then release
that neurotransmitter at the synaptic cleft.
And that neurotransmitter diffuses across
the synaptic cleft or the gap between the
axon terminal and the sarcolemma of the muscle
cell. And then the muscle fibre itself has
enormous junctional folds you can see a lot
folded appearance of the sarcolemma of the
muscle fibre here. That fold appearance is
to increase the surface area of the cell membrane
of the muscle fibre, the sarcolemma so that
it can interact with a lot more neurotransmitter
and make the transmission of the axon potential
to a wave of depolarization very efficient
and bring about contraction.
02:33
Let us continue with the motor innervation
of a skeletal muscle fibre and let me explain
to you what a motor unit is. Motor unit,
is the number of muscle fibres that is innervated
by a single neuron, a single axon. In this
picture, you can see there are a number of
muscle fibres that have been tears down and put
into a dish and the nerve fibre has been stained.
03:07
Here is the dark stain. And this nerve fibre,
this axon is coming down and it branches.
03:12
And you can see the motor endplates or neuromuscular
junctions shown on some of the individual
muscle fibres as a round black stained region.
Well, the motor unit, again is the number of muscle fibres
innervated by one single axon or one single neurone.
In ocular muscles that motor unit is very
small. Also maybe in your fingertips, where you need
very precise control of muscle movement. But
in postural muscles, we do not need that precise
movement. The motor unit can be very very
large, one axon can innervate several hundred
different muscle fibres. So make sure you
are aware of what the motor unit is. Now,
I said earlier that muscle has a special sensory
role as well. It has sensory receptors within
it called the muscle spindle. And again I am
not going to go through the physiology of
the muscle spindle, but I just want to point
out a couple of features about the muscle
spindle shown in this diagram. That you can
see, if you look at a histologic section through
a muscle spindle within the muscle mass when
viewed with an electron or a light microscope.
First of all, the spindle cells are of two
types. These are the special sensory cells.
They're wrapped up by an internal capsule and
filled with fluid. And these spindle cells
are called nuclei bag fibres or nuclear chain
fibres. And if you look at the diagram in
more detail yourself, you will find that the
nuclear bag fibre is named just because the
nuclei is filled or appeared to be clumped
together like a bag of nuclei. Whereas the
nuclear chain fiber, the nuclei are elongated
along the length of the muscle fibre like
a chain. So make sure you see the two in the
diagram. Each of those bag fibres or chain
fibres receives innervation from both the sensory
and motorneurons. And together these special
spindle cells perceive the change in muscle
length or stretch. They are called intrafusal
fibres, intrafusal meaning within the spindle.
05:52
Normal skeletal muscle fibres are termed extrafusal
fibres. So again these special muscle spindles,
measure length or gives the information to
the central nervous system about length or
stretch of the muscle fibre. There is also
the Golgi tendon organ. These Golgi tendon
organs are found at the location of where
the muscles starts to form with the tendon.
06:21
And those Golgi tendon organs measure tension
in the muscle. So the muscle is continuously
sending information to the central nervous
system about change in length or stretch and
also tension. And the central nervous system
can then use that information to tell us about
our position in space, to tell us about the
positions of our limbs. When move my arm around
all that information coming from the muscle
spindle and the Golgi tendon organ gives the
central nervous system the information to
be able to locate our position in space.
07:01
Here is a section of the muscle spindle on the
right hand side and the diagram on the left
just shows you the rather elaborate way in
which the bag fibres and the chain fibres
are innervated. But the section shows you
a section through the spindle, you can see
an outer capsule and you can see some muscle
fibres wrapped together. They are going to
be intrafusal fibres wrapped up by fluid and
they have peripheral nuclei you can see also
in the section.