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Initiation of Translation

by Georgina Cornwall, PhD
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    00:01 So we’ll look at those shortly.

    00:03 So when we start translating messenger RNA, the ribosome is actually not assembled.

    00:09 The small subunit will come together with the large subunit only once we have got messenger RNA and the very first tRNA in place.

    00:19 So let’s look at how that happens.

    00:22 We have our small subunit.

    00:24 We have our messenger RNA and we have a charged tRNA ready to go.

    00:33 Now, interestingly enough, every messenger RNA and every gene will begin with a tRNA that carries methionine.

    00:41 So the first amino acid in every single polypeptide is methionine and that will generally get cut during protein modification, so it may not – the polypeptide chain may not end up with a methionine at the very beginning, but it will start that way because formylmethionine is what the ribosome needs to get things going.

    01:05 So all these components come together and we invest a little bit of ATP and use some initiation factors.

    01:13 Initiation factors are other enzymes that help the messenger RNA and the first formylmethionine tRNA gets arranged on the small subunit.

    01:24 We put a little bit of energy in and the anticodon of the first tRNA binds with the start codon on the messenger RNA and once this happens, we have the initiation complex.

    01:39 All together, the ribosomal large subunit will come along and join the party and we are ready to begin the elongation phase of translation.


    About the Lecture

    The lecture Initiation of Translation by Georgina Cornwall, PhD is from the course Gene Expression.


    Included Quiz Questions

    1. …charged N-formylmethionine tRNA docked on the small subunit of the ribosome.
    2. …un-charged N-formylmethionine tRNA docked on the small subunit of the ribosome.
    3. … charged N-phenylalanine tRNA docked on the small subunit of the ribosome.
    4. …charged alanine tRNA docked on the small subunit of the ribosome.
    5. …charged proline tRNA docked on the small subunit of the ribosome.

    Author of lecture Initiation of Translation

     Georgina Cornwall, PhD

    Georgina Cornwall, PhD


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