Lectures

Sanger Sequencing

by Georgina Cornwall, PhD
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    About the Lecture

    The lecture Sanger Sequencing by Georgina Cornwall, PhD is from the course Genomics.


    Included Quiz Questions

    1. Dideoxynucleotides
    2. Regular DAN nucleotides
    3. Template strand
    4. DNA polymerase
    5. Taq Polymerase
    1. …the incorporation of a chain terminating dideoxynucleotides by DNA polymerase during in vitro DNA synthesis.
    2. …the incorporation of a chain initiating dideoxynucleotides by DNA polymerase during in vitro DNA synthesis.
    3. …the incorporation of a chain terminating deoxynucleotides by DNA polymerase during in vitro DNA synthesis.
    4. …the incorporation of a chain initiating deoxynucleotides by DNA polymerase during in vitro DNA synthesis.
    5. …the incorporation of a chain initiating dideoxy-ribonucleotides by DNA polymerase during in vitro DNA synthesis.
    1. …the lack of 3’-OH group on the ribose moiety of the dideoxynucleotide.
    2. …the lack of 5’-OH group on the ribose moiety of the dideoxynucleotide.
    3. …the lack of 2’-OH group on the ribose moiety of the dideoxynucleotide.
    4. …the lack of 1’-OH group on the ribose moiety of the dideoxynucleotide.
    5. …the lack of 4’-OH group on the ribose moiety of the dideoxynucleotide.
    1. Capillary gel electrophoresis
    2. Chromatogram
    3. Fluorescent dye-labeled ddNTPs
    4. X-ray film
    5. Autoradiograph

    Author of lecture Sanger Sequencing

     Georgina Cornwall, PhD

    Georgina Cornwall, PhD


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