Genome Mapping

by Georgina Cornwall, PhD

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    In the previous course, we also covered genome mapping in which we were analyzing patterns of inheritance by checking out the crossing over frequencies between certain genes that are phenotypically expressed. We can look at those recombination frequencies to estimate the distances, say, between A and B and between B and C. Then we could do a three-point cross where we estimated them between A and C. This was purely by looking at the progeny, the offspring involved in crosses and gave us a relative distance. Now, we have more sophisticated mapping techniques because of DNA sequencing. But before, we used to place these genes relative to each other using cytologic maps, using banding patterns and landmarks within DNA to stack pieces up and to see where they belonged. We used sequence tag sites from one segment of DNA to another segment of DNA. Those tag sites help us line up those pieces. So, you should recall all of that information. Now again, we have the more sophisticated techniques. You’ll see first our Sanger dideoxy. This figure should look very familiar. Then we talked about clone-by-clone sequencing. So, we’re still using this Sanger sequencing but we’re cutting up the genome into thousands of pieces, millions of pieces, maybe even more. We’re lining them up using computer analytics. Then shotgun was more of chopping the whole genome rather than chopping it in pieces and then smaller pieces, really just shot gunning it and chopping the genome randomly into thousands and thousands, millions of pieces and then using more sophisticated computer techniques to add up all those pieces and give us the whole genome sequence. So, the technique of sequencing and genome mapping is becoming less and less expensive. It started out being super expensive like billions of dollars just for one...

    About the Lecture

    The lecture Genome Mapping by Georgina Cornwall, PhD is from the course Introduction to Medical Genetics.

    Included Quiz Questions

    1. Microarrays
    2. Analysis of recombination frequencies of progeny
    3. Physical mapping of genome using sequence tag sites
    4. Sanger sequencing
    5. Shotgun sequencing
    1. Sanger sequencing
    2. Cytologic mapping
    3. Microarrays
    4. Fluorescent in situ hybridization
    5. Enzyme-linked immunosorbent assay
    1. Determining when a gene is expressed
    2. Sequencing genome
    3. Determining start and stop codon of a gene
    4. Determining in which cells a gene is expressed
    5. Determining tag sites

    Author of lecture Genome Mapping

     Georgina Cornwall, PhD

    Georgina Cornwall, PhD

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