Thymidylate Synthase and Recycling of Folates by Kevin Ahern, PhD

About the Lecture

The lecture Thymidylate Synthase and Recycling of Folates by Kevin Ahern, PhD is from the course Purine and Pyrimidine Metabolism. It contains the following chapters:

Thymidylate Synthase
Recycling of Folates

Included Quiz Questions

Which of the following is true regarding thymidylate synthase?

It transfers a carbon from a folate to make dTMP.
It catalyzes the removal of a methyl group from dUMP to form dTMP.
It requires 5-fluorouracil to function.
All of the answers are true.
None of the answers are true.

Which of the following is true regarding dihydrofolate reductase?

It is necessary for folate recycling.
It is inhibited by dihydrofolate.
It is inhibited by tetrahydrofolate.
All of the answers are true.
None of the answers are true.

Which of the following is true regarding methotrexate?

All of the answers are true.
It is an inhibitor of dihydrofolate reductase.
It is used in chemotherapy.
It resembles dihydrofolate.

Which of the following is not true regarding suicide inhibitor?

The suicide inhibitor causes the activation of cancer-causing enzymes and leads to the formation of malignant tissues.
The suicide inhibitor binds to the enzyme by covalent bonding and causes irreversible enzyme inhibition.
The suicide inhibitor is usually a substrate analog.
5-fluorouracil is a suicide inhibitor of the thymidylate synthase enzyme.
Due to its suicide inhibitor nature, 5-fluorouracil is used in the treatment of cancerous tissues.

Which of the following descriptions is correct?

5,10-Methylenetetrahydrofolate - donates a methyl group to convert dUMP to dTMP
Methotrexate - stimulates cell division by participating in regeneration of 5,10-methylenetetrahydrofolate
Thymidylate synthase - converts dTMP to dUMP
5-fluorouracil - promotes the conversion of dUMP to dTMP
Serine hydroxymethyltransferase - promotes the conversion of dihydrofolate into tetrahydrofolate