African Trypanosomiasis: Diagnosis

by Vincent Racaniello, PhD

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    00:00 This is a terrible affliction for which we need really to devise preventatives.

    00:01 How do you diagnose such infections? You can look at blood, you can take a very small amount of blood and simply look at it under the microscope, you can find the trypomastigotes, they are quite beautiful, as I said. They stain very nicely and they’re clear to identify. You can also find such trypanosomes in cerebrospinal fluid, by that time of course the infection has been going on for quite a while and will be very difficult to eliminate. There is a relatively recently developed field PCR test which can be done with minimal equipment, and this PCR test, polymerase chain reaction, is different from the usual PCR test, where you have to amplify the DNA of the parasite and then fractionate the DNA on a gel, to see if the reaction is positive or negative. Here, the PCR test has been developed, such that if the color goes from green to brown, it means that the trypanosome DNA is present.

    01:09 So this is a very nice development that makes it far easier and quicker to carry out PCR in regions with not a lot of equipment.

    About the Lecture

    The lecture African Trypanosomiasis: Diagnosis by Vincent Racaniello, PhD is from the course Parasites.

    Included Quiz Questions

    1. Trypomastigotes
    2. Promastigote
    3. Amastigote
    4. Protomastigote
    5. Schizont
    1. PCR
    2. ELAISA
    3. Microscopy
    4. Antigen antibody test
    5. UV Amplification

    Author of lecture African Trypanosomiasis: Diagnosis

     Vincent Racaniello, PhD

    Vincent Racaniello, PhD

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